CRIP1 (Cysteine Rich Protein 1, Intestinal, CRP1, CRHP, CRIP, Cysteine-rich heart protein, Cysteine-rich intestinal protein)

Size

£626.00

SKU:: 362688
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Pro2-Lys77 of human CRIP1, expressed in E .coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family, members of which include cysteine- and glycine-rich protein-1 (CSRP1), rhombotin-1 (RBTN1), rhombotin-2 (RBTN2), and rhombotin-3 (RBTN3). CRIP may be involved in intestinal zinc transport. The deduced protein, which the authors called cysteine-rich heart protein (CRHP), is 97% identical to mouse Crip. The 77aa CRHP contains a single LIM motif and a glycine-rich domain. Northern blot analysis detected an ~670-bp CRHP mRNA in human fetal heart. Rat Crip was highly expressed in small intestine, with lower levels in adult heart and spleen; the authors found that rat Crip is developmentally regulated in heart. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice