CPA1 (Carboxypeptidase A1, Pancreatic)

Size

£577.00

SKU:: 362653
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Thr205-Tyr419 of rat CPA1, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Carboxypeptidase A1 is a monomeric pancreatic exopeptidase. It is involved in zymogen inhibition. The method allowed the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate the strategy, short diagnostic sequence tags (SSTs) were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1,000 tags revealed a gene expression pattern characteristic of pancreas. New pancreatic transcripts corresponding to novel tags were also identified. SAGE is based on 2 principles: first, that a short nucleotide sequence tag (9 to 10 bp) contained sufficient information to uniquely identify a transcript; and second, that concatenation of SSTs allows the efficient analysis of transcripts in a serial manner by the sequencing of multiple tags within a single clone. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice