362624
COL10 (Collagen Type X, COL10A1, COL10-A1, Schmid Metaphyseal Chondrodysplasia, Collagen Alpha-1(X)chain)
Size
£505.00
- SKU:
- 362624
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ala547-Met674 of bovine COL10 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Collagen alpha-1(X) chain is the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). During development and growth of long bones, chondrocytes pass sequentially through a proliferative, a hypertrophic and a degenerative stage, each characterized by a particular set of collagen types. Proliferative (stage I) chondrocytes synthesize type II collagen as the major collagen and types IX and XI as the minor collagens. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AFTV ILSKAYPAIG TPIPFDKILY NKQQHYDPRT GIFTCKIPGI YYFSYHIHVK GTHAWVGLYK NGTPVMYTYD EYIKGYLDQA SGSAVIDLTE NDQVWLQLPN AGSNGLYSPE YVHSSFSGFL VAPM Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice