362613
CNPY2 (Canopy 2 Homolog, ZSIG9, Cnpy2, MSAP, TMEM4, Transmembrane Protein 4, MIR-interacting saposin-like protein, Putative secreted protein Zsig9)
Size
£517.00
- SKU:
- 362613
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Arg21-Leu182 of human CNPY2 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- TMEM4 encodes a predicted 182aa type II transmembrane protein. The first 3 to 20aa of MSAP constitute the SA region, which is followed by a saposin -like domain interrupted by 2 stretches of 30 and 40aa Northern blot analysis detected MSAP expression in all adult and fetal tissues examined, with highest levels in adult placenta, liver, and pancreas. Western blot analysis of placenta and brain detected MSAP at an apparent molecular mass of 21 kD. Immunofluorescence microscopy showed colocalization of Msap with Mir in COS-7 cells. In cultured rodent primary hippocampal neurons, the 2 proteins localized around the nucleus and extended into the neurites. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- RRSQDLHCGA CRALVDELEW EIAQVDPKKT IQMGSFRINP DGSQSVVEVP YARSEAHLTE LLEEICDRMK EYGEQIDPST HRKNYVRVVG RNGESSELDL QGIRIDSDIS GTLKFACESI VEEYEDELIE FFSREADNVK DKLCSKRTDL CDHALHISHD EL Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice