CNP (2',3'-Cyclic Nucleotide 3'-Phosphodiesterase, 2,3CNP, 2,3-CNP, CNPCNP1, Cyclic-CMP Phosphodiesterase, 2',3'-Cyclic AMP Phosphodiesterase, CNPase, 2':3'-CNMP-3'-ase)

Size

£626.00

SKU:: 362609
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Leu186-Ile421 of human CNP, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: 2,3-CNP is a myelin-associated enzyme that makes up 4% of total CNS myelin protein, and is thought to undergo significant age-associated changes. CNP is expressed exclusively by oligodendrocytes in the CNS, and the appearance of CNP seems to be one of the earliest events of oligodendrocyte differentiation.CNP is thought to play a critical role in the events leading up to myelination. CNP also associates with microtubules in brain tissue and FRTL-5 thyroid cells, and is reported to have Microtubule-associated protein-like activity, being able to catalyze microtubule formation at low molar ratios. Deletion of the C-terminus of CNP or phosphorylation abolish the catalytic activity of microtubule formation. CNP can link tubulin to cellular membranes, and might be involved in the regulation cytoplasmic microtubule distribution. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice