362580
CITED1 (Cbp/p300 Interacting Transactivator, With Glu/Asp Rich Carboxy Terminal Domain 1, MSG1, Melanocyte-specific protein 1)
Size
£626.00
- SKU:
- 362580
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1-Cys193 of human CITED1 expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- MSG1 transcripts in pigmented human epidermal melanocytes but did not detect MSG1 expression in any of the examined depigmented or weakly pigmented human melanoma cells. The deduced 193aa human MSG1 protein shares 75% overall sequence identity with mouse Msg1. The C-terminal 54aa of these proteins are 96% identical and highly acidic, with 22% of the aa being either aspartic acid or glutamic acid; this region forms a predicted amphipathic alpha-helical structure. Immunoprecipitation studies using an antibody against Msg1 detected a 27-kD protein in human epidermal melanocyte lysates. Immunofluorescence cytochemistry localized MSG1 to the nucleus of normal human epidermal melanocytes and human melanoma cells. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MPTTSRPALD VKGGTSPAKE DANQEMSSVA YSNLAVKDRK AVAILHYPGV ASNGTKASGA PTSSSGSPIG SPTTTPPTKP PSFNLHPAPH LLASMHLQKL NSQYQGMAAA TPGQPGEAGP LQNWDFGAQA GGAESLSPSA GAQSPAIIDS DPVDEEVLMS LVVELGLDRA NELPELWLGQ NEFDFTADFP SSC Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice