362575
CIDEC (Cell Death Inducing DFFA Like Effector C, CIDE-3, Fsp27, Fat-specific protein FSP27 homolog)
Size
£533.00
- SKU:
- 362575
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1-Gln221 of rat CIDEC, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- DNA fragmentation factor (DFF) induces the fragmentation of DNA associated with apoptosis. A novel family of cell death-inducing DFF45-like effectors (CIDEs), including CIDEC, can also promote apoptosis. The deduced 238aa protein has a predicted molecular mass of 26.8 kD and shares 44% and 38% identity with CIDEA and CIDEB, respectively. Northern blot analysis detected a 1.3-kb transcript mainly in small intestine, heart, colon, and stomach, with lower expression observed in brain, kidney, and liver. A 1.1-kb transcript was detected in placenta. The authors identified a 164aa CIDEC isoform, which they called CIDE3-alpha, lacking the first N-terminal 74aa. Immunofluorescence analysis localized CIDEC in a punctate manner to the cytosol. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice