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CHAF1B (Chromatin Assembly Factor 1, Subunit B, CAF-1, CAF-IP60, CAF1, CAF1A, CAF1P60, MPHOSPH7, MPP7, M-Phase Phosphoprotein 7, Chromatin Assembly Factor I, p60 subunit, M-phase phosphoprotein 7)

Size

£537.00

SKU:: 362545
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Asn199 of mouse CHAF1B, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: CHAF1B corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. The predicted 559aa protein belongs to the WD (tryptophan-aspartate), or transducin, family. These proteins are generally characterized by 4 to 8 copies of a repeat of approximately 40 residues that often ends with a WD sequence (p60 has 7 copies of the motif). The p60 subunit also has a PEST (proline-glutamic acid-serine-threonine) box at its C terminus that is associated with proteins of short half-lives. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

Shipping Conditions:: Blue Ice