362527
CENTa2 (Centaurin Alpha 2, ADAP2, Cent-b, Arf-GAP With Dual PH Domain-Containing Protein 2)
Size
£577.00
- SKU:
- 362527
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ser49-Ser360 of rat CENTa2, fused to N-terminal Tags, His-Tag and T7-tag expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- CENTA2 contains an N-terminal ARF-GAP -like zinc finger motif, followed by 2 pleckstrin homology (PH) domains. Northern blot analysis detected highest expression in placenta, spleen, kidney, skeletal muscle, and adrenal gland, with weaker expression in thyroid, liver, heart, lung, small intestine, and peripheral blood leukocytes. CENTA2 was localized exclusively at the plasma membrane of transfected embryonic kidney cells. CENTA2 expressed in insect cells bound inositol 1,3,4,5-tetrakisphosphate and phosphatidylinositol 3,4,5-trisphosphate with similar affinities. Modeling studies suggested that there is only 1 class of binding sites, and stoichiometric analysis indicated that there are 2 binding sites per CENTA2 molecule. Inhibition of PI3K caused membrane-bound CENTA2 to dissociate into the cytosol. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SG VHRNFPDISK VKSVRLDFWD DSMVEFMTHN GNLSVKAKFE ARVPTFYYVP QASDCLVLKE QWIRAKYERQ EFMAEKAVSP PGDREGFLWK RGRDNSQFLR RRFVLLSREG LLKYYTKEEG KTPKAIINIK DLNATFQTEK IGHPHGLQIT YRKEGQTRNL FVYHDSGKEI VDWFNALRAA RLQYLKLAFP DLPESELVPL ITRNYLKQGF MEKTGPKHRE PFKKRWFALD PQERRLLYYK NPLDAFEQGQ VFLGSNEQGY EVWEGLPQGI RGNRWKVGLT VITPERKFVF TCPTEKEQRE WLESLRGVLS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice