CDKN2A (Cyclin Dependent Kinase Inhibitor 2A, P16, ARF, CDK4I, CMM2, INK4, INK4a, MLM, MTS1, TP16, P14, P14ARF, P16INK4a, P19, Melanoma P16, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1)

Size

£495.00

SKU:: 362507
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ser3-Pro155 of rat CDKN2A, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: CDKN2A, is a tumor suppressor protein, which in humans is encoded by the CDKN2A gene. P16 plays an important role in regulating the cell cycle, and mutations in p16 increase the risk of developing a variety of cancers, notably melanoma. This gene generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-1:400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SSADRLAR AAALGREHEV RALLEAGASP NAPNTFGRTP IQVMMMGNVK VAALLLSYGA DSNCEDPTTL SRPVHDAARE GFLDTLVVLH QAGARLDVRD AWGRLPLDLA LERGHHDVVR YLRYLLSSAG NVSRVTDRHN FCSSTPRCLG LRGQP Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice