CDA (Cytidine Deaminase, CDD, Cytidine aminohydrolase)

Size

£626.00

SKU:: 362479
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa2-146 of human CDA, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Cytidine deaminase forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias. Kuhn et al. (1993) identified a cDNA clone corresponding to the human CDA gene in a study of expression products related to monocyte and macrophage differentiation. The deduced 145-residue protein has a molecular mass of 16.1 kD and shows nearly 50% homology to cytidine deaminase from Bacillus subtilis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-1:400 Immunocytochemistry: 1:50-1:500 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (paraffin): 1:10-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice