CD6 (Cluster Of Differentiation 6, TP120, T12, T-cell differentiation antigen CD6)

Size

£495.00

SKU:: 362468
Application:: WB
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ser477-Pro644 of rat CD6, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: CD6 is a monomeric 105- or 130kD membrane glycoprotein that is involved in T-cell activation. The size difference between the 2 CD6 forms is due to differences in phosphorylation. By screening a human peripheral blood acute lymphocytic leukemia cell cDNA expression library with antibodies against CD6, Aruffo et al. (1991) cloned a cDNA encoding CD6. The predicted 468aa protein contains a 24aa signal sequence, 3 extracellular 'scavenger receptor cysteine-rich' (SRCR) domains, a membrane-spanning domain, and a 44aa cytoplasmic domain. It shows significant homology to CD5. By Northern blot analysis, CD6 is expressed as an approximately 3-kb mRNA in T cells. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.