362457
CD276 (Cluster Of Differentiation 276, B7-H3, B7H3, CD276 Molecule, B7 homolog 3, Costimulatory molecule)
Size
£482.00
- SKU:
- 362457
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa47-219 of human CD276 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- Sequence analysis predicted that the 316aa type I transmembrane protein contains an N-terminal signal peptide; extracellular V- and C-like Ig domains with 4 conserved cysteine residues; a transmembrane region; and a 45aa cytoplasmic tail. The extracellular receptor- binding domain of B7H3 shares 27% and 25% identity with those of B7H1 and B7H2, respectively. Northern blot analysis detected highest expression of a 4.1-kb transcript in heart, liver, placenta, prostate, testis, uterus, pancreas, small intestine, colon, and lymphoid organs, with lower expression in brain, skeletal muscle, kidney, and lung, and no expression in peripheral blood leukocytes. B7H3 expression was detected in most tumor cell lines tested. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 0.01-3ug/ml Immunohistochemistry (Paraffin): 5-30ug/ml Flow Cytometry: 0.5-2ug/10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice