CASP8 (Caspase 8, CAP4, FLICE, MACH, MCH5, Cysteinyl Aspartate Specific Proteinases 8, Apoptotic cysteine protease, FADD-homologous ICE/ced-3-like protease, MORT1-associated ced-3 homolog)

Size

£524.00

SKU:: 362415
Purity:: Purified by Protein A and peptide affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa7-21 of human CASP8 expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Caspase 8 is a member caspase family. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 0.01-3ug/ml Immunohistochemistry: 5-30ug/ml Immunocytochemistry (IF-IC): 5-30ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice