362401
CARNS1 (Carnosine Synthase 1, ATPGD1, ATP-Grasp Domain Containing 1)
Size
£537.00
- SKU:
- 362401
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa623-826 of mouse CARNS1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- CARNS1, a member of the ATP-grasp family of ATPases, catalyzes the formation of carnosine and homocarnosine, which are found mainly in skeletal muscle and the central nervous system, respectively. The deduced proteins contain 957aa and 950aa, respectively, and both contain 2 ATP-grasp domains, with highest conservation in the C-terminal catalytic domain. Gel filtration analysis showed that mouse Atpgd1, like the chicken enzyme, was expressed as a homotetramer of about 430kD in transfected HEK293 cells.Mouse and human ATPGD1 catalyzed the same reaction following expression in HEK293 cells. All 3 enzymes were not specific with respect to the aa serving as the acceptor, although L-histidine was preferred, and all 3 were also more efficient in synthesizing carnosine than homocarnosine and other dipeptides. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.5-2ug/ml Western Blot: 5-20ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice