CAPN1 (Calpain 1, Large Subunit, PIG30, CANPL1, MuCANP, MuCL, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium- activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain)

Size

£524.00

SKU:: 362392
Application:: WB
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Lys426-Val676 of human CAPN1 fused to His-Tag at N-terminal expressed in E. coli
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Calpain (calcium-dependent protease) is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80kD) and S (small, regulatory, 30kD) subunits. The isozymes share an identical S subunit (CAPNS1), with the differences arising from the L subunits, L1 (CAPN1) and L2 (CAPN2). The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. Calpain-1 catalytic subunit is the large subunit of the ubiquitous enzyme, calpain 1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice