CA6 (Carbonic Anhydrase VI, CA-VI, CAVI, GUSTIN, Carbonate dehydratase VI, Salivary carbonic anhydrase, Secreted carbonic anhydrase)

Size

£577.00

SKU:: 362361
Application:: WB
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Thr147-Ala275 of rat CA6 expressed in E. coli
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Carbonic anhydrase 6 is one of several isozymes of carbonic anhydrase. This protein is found only in salivary glands and saliva and protein may play a role in the reversible hydratation of carbon dioxide though its function in saliva is unknown. The clones identified a 1.45-kb mRNA that was present in high levels in parotid submandibular salivary glands but absent in other tissues such as sublingual gland, kidney, liver, and prostate. The mature CA VI protein has 291aa, compared to the 259 or 260 residues of the cytoplasmic isozymes; most of the extra aa present are in the C-terminal region. The CA VI protein has 35% sequence identity with human CA II, while residues involved in the active site of the enzymes have been conserved. Southern analysis of human DNA indicated that there is only 1 gene coding for CA VI. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TYEK AVDQPDGLAV MAVLVKVEDY TENDYYSTFI SELENVKYTG QTTTLRNVNI RNMLPGDIRH YYTYQGSLTT PPCTENVKWF VFQDSATISK AQAERIENAV MDHHNQTIRN GYRRTQPLHN RVVEA Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice