362358
C9 (Complement Component 9)
Size
£524.00
- SKU:
- 362358
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gln22-His265 of human C9 expressed in E. coli
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Activation of the complement system results in formation of the membrane attack complex (MAC) on the membranes of target cells. The complex is assembled by sequential addition of 1 molecule each of C5b, C6, C7, and C8 and 6 to 16 molecules of the ninth component, C9. DiScipio et al. (1984) screened a human liver cDNA library by the colony-hybridization technique using 2 radiolabelled oligonucleotide probes that correspond to known regions of the C9 aa sequence. The cDNA coding for C9 was sequenced and the protein sequence--537aa in a single polypeptide chain-was derived. The N-terminal half of C9 is predominantly hydrophilic and the C-terminal half is more hydrophobic. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QYTTSYDPE LTESSGSASH IDCRMSPWSE WSQCDPCLRQ MFRSRSIEVF GQFNGKRCTD AVGDRRQCVP TEPCEDAEDD CGNDFQCSTG RCIKMRLRCN GDNDCGDFSD EDDCESEPRP PCRDRVVEES ELARTAGYGI NILGMDPLST PFDNEFYNGL CNRDRDGNTL TYYRRPWNVA SLIYETKGEK NFRTEHYEEQ IEAFKSIIQE KTSNFNAAIS LKFTPTETNK AEQCCEETAS SISLH Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice