C6 (Complement Component 6)

Size

£626.00

SKU:: 362353
Application:: WB
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Gln682-Ala934 of human C6, expressed in E. coli
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC (DiScipio and Hugli, 1989). The deduced C6 protein contains 934aa, including a 21aa N-terminal signal sequence and 2 N-glycosylation sites. The mature 913aa C6 protein shares 29% aa identity with C7, and 55 of 56 cysteines in C7 match those in C6. The C6 polypeptide chain is cross-linked by 32 disulfide bonds, and most cysteines are located in short, discrete segments that exhibit homology with a variety of proteins, including thrombospondin, low density lipoprotein receptor(LDLR), epidermal growth factor (EGF), and complement factors H (CFH) and I (CFI ). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.