362344
C4 (Complement Component 4, C4BP-a, Complement Component 4 Binding Protein, Alpha)
Size
£495.00
- SKU:
- 362344
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Leu1428-Gln1660 of chicken C4, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Complement component 4 is a protein involved in the complement system.It is cleaved into proteins 4a and 4b. C4d is the degradation product of the activated complement factor C4, a component of the classical complement cascade which is typically initiated by binding of antibodies to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed which allow transient, covalent binding of the degradation product C4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. C4d is also found in intracytoplasmic vacuoles of endothelial cells. Covalent binding renders C4d a stable molecule that can easily be detected by immunohistochemistry. Detection of C4d is regarded as an indirect sign, a ‘footprint’ of an antibody response. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LRD VVDHWISHYE LEGNQLVLYL DEVPPERQCL SFGATQDAAV GHMQPAMAAI YDYYEPGQRC TVFYNAPQRS STIATLCSPK ICECAQGGCP QAQRRTQEVT ADDRHDFACY SPRVDYALVV RVLSQSEIGA FVAFETEIKE VLLEGQDTAV APGERRRLLV RKSCPLRLQL HNIYLVMGGS GRTRDPEGRP QFLLGPHSWL EEVPSPGRCK ATRLRGYCAQ LQEFRTRLSQ Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice