362343
C3c (Complement C3 Convertase, C4b2a)
Size
£581.00
- SKU:
- 362343
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to mouse C3c expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin, 50% glycerol.
- Extra Details:
- There are two forms of C3-convertase (EC 3.4.21.43). The first is an enzyme composed of the C4b-C2a complex, which forms during the Classical pathway of the complement system. It is formed when C1s cleaves off C2b from a membrane-bound C4b-C2a complex. The second consists of the C3bBb complex, which forms during the alternative complement pathway. It is formed when membrane bound C3b binds to factor B which is in turn cleaved by factor D into Ba and Bb. Bb remains bound to C3b while Ba is released into the surrounding medium. Soluble C3-convertase, also known as C4b2a, catalyzes the proteolytic cleavage of C3 into C3a and C3b as part of the classical complement system as well as the mannan-binding lectin pathway. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice