362330
C1qA ((Complement Component 1, Q Subcomponent A, q Subcomponent, Alpha Polypeptide)
Size
£577.00
- SKU:
- 362330
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Glu23-Ala245 of rat C1qA expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- Reid (1974) reported a partial aa sequence of 95 residues of the 191 residues in the oxidized A chain of human subcomponent C1q. This region of the A chain contains a repeating sequence of glycine-X-Y, where X is often proline and Y is often hydroxyproline, for 78 residues. The 5 hydroxylysine residues and 5 hydroxyproline residues in the oxidized A chain are all in these 78 residues and only in the Y position of the repeating sequence.Prolonged collagenase digestion of the oxidized A chain yielded a large, apparently C-terminal peptide containing most of the noncollagenous sequences present in the chain. Reid (1974) concluded that the A chain of C1q contains a collagen-like region that constitutes most of the N-terminal half of the chain. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EDVCRAPN GKDGVAGIPG RPGRPGLKGE RGEPGAAGIR TGIRGLKGDM GESGPPGKPG NVGFPGPTGP LGNSGPQGLK GVKGNPGNIR DQPRPAFSAI RQNPPTYGNV VVFDKVLTNQ ENPYQNRTGH FICAVPGFYY FTFQVISKWD LCLSIVSSSR GQPRNSLGFC DTNSKGLFQV LAGGTVLQLQ RGDEVWIEKD PAKGRIYQGT EADSIFSGFL IFPSA Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice