362322
BRD1 (Bromodomain Containing Protein 1, BRL, BRPF2, BR140-like protein, Bromodomain and PHD finger-containing protein 2)
Size
£626.00
- SKU:
- 362322
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gln579-Asp1058 of human BRD1 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The MLL is reciprocally translocated with 1 of a number of different partner genes in human acute leukemias. The precise mechanism of oncogenic transformation is unclear since most of the partner genes encode unrelated proteins. However, 2 partner genes, AF10 and AF17, are related through the presence of a cysteine-rich region and a leucine zipper. Overall, the protein was most closely related to the BR140 protein, and was therefore designated BRL (BR140-like gene). Northern blot analysis revealed that a 4.6-kb BRL transcript is expressed at high levels in testis and in several cell lines. A monoclonal antibody raised to a BRL peptide sequence confirmed its widespread expression as a 120kD protein and demonstrated localization to the nucleus of spermatocytes. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice