362313
BOC (Brother Of CDO Homolog)
Size
£522.00
- SKU:
- 362313
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Lys668-Thr866 of mouse BOC, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The 1113aa BOC protein contains 4 Ig repeats, followed by 3 FNIII repeats, a single-pass transmembrane domain, and a 238-residue intracellular region. The domain structure of BOC is similar to that of CDON, and the 2 proteins share 38 to 80% amino acid identity in the individual extracellular domains. The intracellular region of BOC, however, is unrelated to that of CDON. In situ hybridization analysis revealed that, like Cdon, Boc was strongly expressed in dorsal neural tube, somites, and dermomyotomes during murine embryonic development. Northern blot analysis detected a 5-kb transcript in myoblasts in the absence of Ras (190020) transformation. BOC was expressed in most adult human tissues tested, with highest levels in skeletal muscle and small intestine. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice