BLVRB (Biliverdin Reductase B, FLR, SDR43U1, Flavin Reductase,NADPH, Short Chain Dehydrogenase/Reductase Family 43U, Member 1, Biliverdin-IX beta-reductase, Green heme-binding protein)

Size

£626.00

SKU:: 362290
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ala2-Gln206 of human BLVRB, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B. BLVRB is found predominantly in fetal liver. BLVRA is a major component of human adult liver and is identical to the enzyme previously reported as biliverdin reductase. The FLR cDNA, which appeared to be identical to the BLVRB cDNA, encodes a deduced 206aa protein. Western blot analysis of rat tissues showed highest levels of FLR in the erythrocytes and liver. Northern blot analysis detected a 1.1-kb transcript in human tissues. that flavins are the effective electron acceptor of the enzyme and purified FLR to homogeneity. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice