BLVRA (Biliverdin Reductase A, BLVR, BVR, Biliverdin-IX alpha-reductase)

Size

£626.00

SKU:: 362289
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met-His125 of human BLVRA, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Biliverdin reductases, such as BLVRA (EC 1.3.1.24), catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH. The deduced 296aa protein has a calculated molecular mass of 33.2kD. Removal of 2 N-terminal amino acids results in a 294aa mature protein with an N-terminal threonine. The N-terminal region contains the NADH/NADPH-binding consensus sequence. BLVRA shares 82.8% amino acid identity with rat Blvra, but it does not share significant homology with BLVRB. Northern blot analysis detected BLVRA at 1.35 kb in all 8 tissues examined. Expression was highest in brain, pancreas, and lung, and lowest in liver and placenta. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice