BCAN (Brevican, BEHAB, CSPG7, Chondroitin Sulfate Proteoglycan 7, Brevican Proteoglycan, Brevican core protein, Brain-enriched hyaluronan-binding protein)

Size

£537.00

SKU:: 362258
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa23-353 of mouse BCAN, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Neurocan is a developmentally regulated CSPG that shares functional domains with the cartilage CSPG aggrecan and the fibroblast CSPG versican. Yamada et al. (1994) described the molecular cloning of a novel bovine brain proteoglycan of the aggrecan/versican/neurocan family. Since it is the shortest core protein in this family, they named it brevican (from the Latin word 'brevis,' meaning 'short'). A significant amount of brevican devoid of any glycosaminoglycan chains is present in brain, indicating that brevican is a 'part-time' proteoglycan. Northern blot analysis demonstrated that a single 3.3-kb brevican transcript is present predominantly in the brain and that it is expressed in primary cerebellar astrocytes but not in neurons. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-3ug/ml Immunohistochemistry (Paraffin): 5-30ug/ml Immunocytochemistry: 5-30ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice