362217
ATF7 (Activating Transcription Factor 7, ATFA, Cyclic AMP-Dependent Transcription Factor ATF-7)
Size
£495.00
- SKU:
- 362217
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Gln50-Ser270 of mouse ATF7, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Activating transcription factors (ATFs), or cAMP response element-binding proteins (CREBs), bind to cAMP-inducible promoters and are involved in gene transcription. By screening a HeLa cell cDNA library using an adenovirus ATF-binding sequence as the probe, Gaire et al. (1990) isolated cDNAs encoding ATF7, which they called ATFA, and a splice variant. The predicted 483aa full-length phosphoprotein contains a leucine zipper preceded by a region rich in positively charged aa and is homologous to ATF2. Compared with full-length ATF7, the splice variant lacks residues 114 to 134. atf7b is maternally expressed and during embryogenesis its mRNA is ubiquitously distributed, showing an expression pattern similar to that of mammalian Atf7. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- Q TPTPTRFLKN CEEVGLFNEL ASSFEHEFKK ASDDDEKKGA AGPLDMSLPS TPDIKIKEEE PVEVDSSPPD SPASSPCSPP LKEKEVTTKP VVISTPTPTI VRPGSLPLHL GYDPLHPTLP SPTSVITQAP PSNRQIGSPT GSLPLVMHLA NGQTMPMLPG PPVQMPSVIS LARPVSMVPN IPGIPGPPVN NSGSISPSGH PMPSEAKMRL KATLTHQVSS Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice