362193
ASCL1 (Achaete Scute Complex Like Protein 1, ASH1, HASH1, MASH1, BHLHA46, Achaete Scute Homolog 1, Class A basic helix-loop-helix protein 46)
Size
£626.00
- SKU:
- 362193
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Lys84-Phe236 of human ASCL1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Mash1 is expressed during development of rat retina and interacts specifically with an E-box identified in the promoter for the opsin gene during rod photoreceptor differentiation. It encodes a predicted protein of 238aa that shares 95% identity with the mammalian achaete-scute homolog-1 (Mash1), a rodent basic helix-loop-helix factor. The proximal coding region of the cDNA contains a striking 14-copy repeat of the triplet CAG that exhibits polymorphism in human genomic DNA; thus, ASCL1 is a candidate locus. Northern blot analysis revealed ASCL1 transcripts in RNA from a human MTC cell line, 2 fresh MTC tumors, fetal brain, and 3 lines of human SCLC. In contrast, cultured lines of non-SCLC lung cancers and a panel of normal adult human tissues showed no detectable ASCL1 transcripts. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice