APOA4 (Apolipoprotein A4, ApoA-IV, ApoAIV, Apo-A4)

Size

£523.00

SKU:: 362152
Application:: WB
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Glu21-Gly380 of bovine APOA4, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: APOA4 resides on chromosome 11 in close linkage to APOA1 and APOC3. APOA4 contains 3 exons separated by two introns, and is polymorphic, although most of the reported sequence polymorphisms occur in exon 3. The best validated and studied non-synonymous SNPs are a glutamine=> histidine substitution at codon 380 and a threonine=> serine substitution at codon 367; a sequence polymorphism has also been identified in the 3'UTR of the third exon. Intra-species comparative gene sequence analysis suggests that the APOA4 gene arose from APOA1 by gene duplication approximately 270 MYA. The primary translation product of the APOA4 gene is a 396-residue preprotein, which undergoes proteolytic processing to yield apo A-IV, a 376-reside mature O-linked glycoprotein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-EVNADQVATV IWDYFSQLGN NAKKAVEHIQ KSELTQQLNT LFQDKLGEVS TYTDDLQKKL VPFATELHER LTKDSEKLKE EIRKELEDLR ARLLPHATEV SQKIGDNVRE LQQRLGPYAE ELRTQVDTQA QQLRRQLTPY VERMEKVMRQ NLDQLQASLA PYAEELQATV NQRVEELKGR LTPYADQLQT KIEENVEELR RSLAPYAQDV QGKLNHQLEG LAFQMKKHAE ELKAKISAKA EELRQGLVPL VNSVHGSQLG NAEDLQKSLA ELSSRLDQQV EDFRRTVGPY GETFNKAMVQ QLDTLRQKLG PLAGDVEDHL SFLEKDLRDK VSSFFNTLKE KESQAPALPA QEEMPVPLGG Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice