ALOX15B (Arachidonate-15-Lipoxygenase, Type B, LOX15B, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Lipoxygenase 15, Type B, 15-lipoxygenase 2, Linoleate 13-lipoxygenase 15-LOb)

Size

£524.00

SKU:: 362086
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Arg253-Glu490 of human ALOX15B, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Arachidonate 15-lipoxygenase type II is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. When expressed in mammalian cells, ALOX15B converted arachidonic acid exclusively to 15S-hydroperoxide, while linoleic acid was a relatively poor substrate. Northern blot analysis detected expression of a 2.5- to 3-kb ALOX15B mRNA in lung, cornea, and prostate. The previously characterized ALOX15 has distinct catalytic activities and is strongly expressed in specific blood cell types. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice