362071

AKAP11 (A Kinase Anchor Protein 11, AKAP220, PRKA11, Protein Kinase A Anchoring Protein 11, A Kinase(PRKA)Anchor Protein 11, A-kinase anchor protein 220 kDa, Protein kinase A-anchoring protein 11)

Size

£626.00

SKU:: 362071
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Asp159-Lys258 of human AKAP11, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: A-kinase anchor protein 11 is expressed at high levels throughout spermatogenesis and in mature sperm. It binds the RI and RII subunits of PKA in testis. It may serve a function in cell cycle control of both somatic cells and germ cells in addition to its putative role in spermatogenesis and sperm function. The AKAP11 protein has 1129aa and contains a PKA-binding region and a peroxisome targeting motif. Immunohistochemical analysis of rat testis cells showed that AKAP220 and a proportion of RII PKA are colocalized in microbodies that appear to be a subset of peroxisomes. By RT-PCR analysis, Ishikawa et al. (1998) detected expression of human AKAP11 in heart, brain, lung, liver, kidney, testis, and ovary, with weaker expression in skeletal muscle, pancreas, and spleen. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice