362070
AK3 (Adenylate Kinase 3, AK3L1, AK6, AKL3L, Adenylate Kinase 3 Alpha-Like 1, GTP, AMP Phosphotransferase AK3, Mitochondrial)
Size
£626.00
- SKU:
- 362070
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Leu8-Pro227 of human AK3, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- The deduced 227aa protein has a calculated molecular mass of 25.6kD. AK3 shares 57.4% amino acid identity with AK4 (AK3L1). It shares 75%, 90%, and 91% amino acid identity with bovine, rat, and mouse Ak3, respectively. Northern blot analysis detected a 3.2-kb transcript in all tissues examined. Expression was highest in heart, liver, and skeletal muscle, moderate in kidney and pancreas, and weak in brain, placenta, and lung. Transcripts of 5, 1.8, and 1.35 kb were detected in heart, liver, and pancreas. Western blot analysis detected AK3 at an apparent molecular mass of 28kD in all tissues examined. Subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction. Human embryonic kidney cells had endogenous phosphotransferase activity, which increased following transfection of AK3 cDNA. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice