362014

Adenylate Cyclase 4 (ATP pyrophosphate-lyase 4, Adenylate cyclase type IV)

Size

£495.00

SKU:: 362014
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ile352-Val541 of mouse ADCY4, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Adenylate cyclase type 4 is a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Mouse studies show that adenylate cyclase 4, along with adenylate cyclases 2 and 3, is expressed in olfactory cilia, suggesting that several different adenylate cyclases may couple to olfactory receptors and that there may be multiple receptor-mediated mechanisms for the generation of cAMP signals. By database analysis and PCR of a human trabecular bone cell cDNA library, Ludwig and Seuwen (2002) identified full-length ADCY4. The deduced protein contains 1077aa. Semiquantitative RT-PCR detected ubiquitous expression of ADCY at moderate levels. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IRKLRVATG VDINMRVGVH SGSVLCGVIG LQKWQYDVWS HDVTLANHME AGGVPGRVHI TGATLALLAG AYAVERADTE HRDPYLRELG EPTYLVIDPR AEEEDEKGTA KGLLSSLEGH TMRPSLLMTR YLESWGAAKP FAHLSHLDSP VSTSTPLPEK AFSPQWSLDR SRTPRGLDDE LDTGDAKFFQ V Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice