ADAM8 (A Disintegrin And Metalloprotease 8, CD156-A, MS2, CD156a, Disintegrin and metalloproteinase domain-containing protein 8)

Size

£482.00

SKU:: 361998
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ser371-Glu587 of human ADAM8, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: ADAM8 is expressed in macrophages, granulocytes, monocytes and B cells. It is an osteoclast stimulating factor. ADAM8 may play a role in neuron-glia interactions during neurodegeneration. It may also mediate extravasation of leukocytes either by degrading the vascular basement membrane or by liberating active molecules from their precursors harbored in the cell surface of leukocytes and endothelial cells. Human ADAM8 precursor consists of 824aa residues consisting of a 16aa signal peptide, a 637aa ectodomain including pro-, metalloprotease, disintegrin-like and cysteine-rich regions, a 25aa transmembrane domain, and a 146aa cytoplasmic domain). ADAM8 naturally occurs as both a membrane and a soluble form. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-SFPRMFSDCS QAYLESFLER PQSVCLANAP DLSHLVGGPV CGNLFVERGE QCDCGPPEDC RNRCCNSTTC QLAEGAQCAH GTCCQECKVK PAGELCRPKK DMCDLEEFCD GRHPECPEDA FQENGTPCSG GYCYNGACPT LAQQCQAFWG PGGQAAEESC FSYDILPGCK ASRYRADMCG VLQCKGGQQP LGRAICIVDV CHALTTEDGT AYEPVPE Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice