361982
Actin Alpha 2, Smooth Muscle (ACT-A2, ACTSA, ACTVS, Asma, GIG46, Cell growth-inhibiting gene 46 protein)
Size
£508.00
- SKU:
- 361982
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa167-377 of human ACTa2, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Actin, aortic smooth muscle is one of six different actin isoforms which have been identified. Six different actin isoforms have been identified in vertebrates by amino acid sequencing: skeletal muscle, cardiac muscle, 2 smooth muscle (enteric and aortic), and 2 cytoplasmic (beta and gamma) (Vandekerckhove and Weber, 1979). Their amino acid sequences are similar and well conserved in evolution; e.g., skeletal and cardiac actins differ by only 4aa, and skeletal muscle and cytoplasmic beta-actins differ by only 25aa out of a total of 374aa. Ueyama et al. (1984) isolated and characterized the human aortic smooth muscle actin gene. The gene has a transition point mutation in position 309, substituting thymine for cytosine. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.01-2ug/ml Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice