361966
ACAT (Acetyl Coenzyme A Acetyltransferase 1, THIL, ACAT, T2, MAT, Acetoacetyl Coenzyme A Thiolase)
Size
£537.00
- SKU:
- 361966
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ala31-Leu424 of mouse ACAT, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. The 427aa precursor had a molecular mass of 45.2 kD. The sequence included a 33-residue leader peptide and a 394aa subunit of the mature enzyme, which had a molecular mass of 41.1kD. In all 4 cell lines, the T2 mRNA had the same 1.7-kb transcript as that of the control; however, content was reduced in 2 cell lines and normal in the other 2. Human T2 is a homotetramer of 41-kD subunits. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice