ABCC10 (ATP Binding Cassette Transporter C10, MRP7, SIMRP7, Multidrug Resistance Associated Protein 7)

Size

£517.00

SKU:: 361957
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Val1246-Pro1492 of human ABCC10, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Members of the ABC family of membrane proteins, such as ABCC10, are involved in energy-dependent transport of a wide variety of substrates across membranes. The 1,492aa ABCC10 protein has a calculated molecular mass of 162kD. It contains 2 nucleotide-binding folds, 17 predicted transmembrane segments arranged in 3 transmembrane domains, and 3 predicted N-glycosylation sites. Northern blot analysis was unable to detected ABCC10 expression, but RT-PCR detected ABCC10 expression in all 10 tissues examined. In vitro-translated ABCC10 had an apparent molecular mass of 158kD by SDS-PAGE. Basal MRP7 promoter activity relied upon a proximal segment of the 5-prime flanking region bearing an E2F site acting cooperatively with 2 closely positioned SP1 sites. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice