361906

IPNV (Infectious Pancreatic Necrosis Virus)

Size

£745.00

SKU:: 361906
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified IPN virus.
Clone:: 1A1/E9
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: IPNV is a single-shelled, non-enveloped virus that belongs to the family Birnaviridae. Its genoma comprises a bisegmented double-stranded RNA, the segment B encodes VP1 and the segment A yields a polyprotein, pVP2-VP4-VP3 and a small protein (VP5) dispensable for viral replication. The polyprotein is autocatalytically cleaved by the endoprotease VP4 to release the structural proteins pVP2 and VP3. IPNV is responsible for infectious pancreatic necrosis, a contagious fish disease characterized by severe damage to the internal organs and tissues with a high mortality among salmonids. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:500 ELISA: 1:5000 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.