His-Tag, CT (Polyhistidine Tag) (Chimeric)

Size

£840.00

SKU:: 361642
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Human
Immunogen:: Purified His-tagged Fab fragment.
Clone:: 3D5 (recomb)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.02% Proclin-300.
Extra Details:: Recombinant monoclonal antibody to His tag (C-terminal) with variable regions (i.e., specificity) from the hybridoma 3D5. This is a chimeric human antibody, based on the original mouse IgG2b format. Manufactured using a proprietary recombinant platform technology. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. Applications: Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.