SbmA (Peptide Antibiotic Transporter SbmA)

Size

£1,068.00

SKU:: 353437
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to SbmA.
Format:: Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide.
Extra Details:: The SbmA protein is a homodimeric secondary transporter protein, that is involved in the transport of microcins B17 and J25, glycopeptide antibiotic bleomycin, proline-rich antimicrobial peptides of prokaryotic and eukaryotic origin, antisense peptide phosphodiamidate morpholino oligomers and peptide nucleic acids (PNA) into the E. coli cytoplasm. This protein is found in a large number of Gram-negative and also Gram-positive microorganisms, including plant and animal pathogens. The monomeric SbmA protein contains 406aa (about 50kD) and has 8 transmembrane regions. The homodimeric complex present in the membrane closely resembles the membrane-spanning region of the ATP-binding cassette transporter family. Peptide transport mediated by SmbA is driven by a electrochemical gradient. SbmA-mediated internalization of peptide substrates suggest that the transport of an unknown substrate(s) represents the function of this protein. Applications: Suitable for use in Western Blot amd Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: The band size to be expected is ~37kD under reducing conditions. Optimal dilutions to be determined by the researcher. Positive Control: Recombinant SbmA or E. coli lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice