CLEC4E (Mincle, Marcophage Inducible C-type Lectin, CLR, CLECSF9)

Size

£876.00

SKU:: 353413
Application:: Flow Cytometry
Purity:: Dialyzed
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat CLEC4E-Fcgamma2b fusion protein.
Clone:: WEN43
Format:: Supplied as a liquid in PBS, 0.1% BSA.
Extra Details:: This receptor is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. For instance, Mincle is involved in mediating inflammatory responses to necrotic cells and is a pathogen sensor for Mycobacterium tuberculosis. It is a member of the Dendritic Cell Inhibitory Receptor (DCIR) group of C-type lectin receptors (CLRs), which are expressed mainly by antigen presenting cells and are encoded in a cluster in the natural killer gene complex. CLRs play crucial roles in defense against pathogens and recognize a variety of carbohydrate moieties. Ligand binding to CLRs leads to the activation of NF-kB via a Syk/CARD9 signaling module. Mincle was originally identified as a lipopolysaccharide-inducible protein in macrophages and has been shown to stimulate inflammatory responses to fungi, although the ligands and signaling pathways involved have not been defined. Mincle does not bind to other receptors in the rat APLEC family. Applications: Suitable for use in Flow Cytometry and Functional Studies. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50, stains the extracellular domain of Mincle transfected 293 cells Optimal dilutions to be determined by the researcher. Positive Control: BW5417 or 293T cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice