Arginase 1 (ARG1)

Size

£819.00

SKU:: 353402
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Peptide corresponding to D14967-1-1t/m6
Clone:: D1.2
Format:: Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide.
Extra Details:: The highest concentration of the enzyme is present in the liver in which the bulk of ureagenesis occurs. Two types of arginases are known: Arginase I and II. The cytosolic enzyme found primarily in liver is Arginase I, a 35kD protein that circulates as trimer. Arginase II is exclusively located in the mitochondrion. Arginase I is next to the liver in man also expressed by mature fetal and adult red blood cells and activated monocytic cells. During inflammation induction of Arginase I by inflammatory cytokines in monocytic cells is considered to lead to a local depletion of arginine resulting in a microenvironment that prevents nitric oxide production and arginine dependent T cell function. Arginase II is expressed by kidney, nucleated red blood cells, brain, spinal cord, gastro-intestinal tract, mammary gland and prostate. Enhanced circulating Arginase I levels have been reported after surgery, following haemorrhage and in asthmatic patients. Measurement of circulating Arginase I has been used experimentally as rapid marker for liver injury. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2ug stains intracellular Arginase 1. For intracellular staining 250,000 cells were permeabilized with buffer containing 0.1% Saponin. The HepG2 cells were fixed in 4% paraformaldehyde before staining. ELISA: can be used as a coat antibody in immune assay setting. Western Blot: A reduced sample treatment and SDS-Page was used. The band size is 40kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice