Amyloid beta, NT (Abeta, beta-Amyloid)

Size

£819.00

SKU:: 353401
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa1-17 from Amyloid beta at N-terminal.
Clone:: VU-17
Format:: Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide.
Extra Details:: Amyloid beta peptides are of key importance in the development of Alzheimer’s disease (AD) and are present in CSF. AD affects ~1% of the population over the age of 80. The disease is characterized by aggregation of Abeta into oligomers, fibrils and plaques deposition of insoluble and aggregated protein in the brain where its neurotoxic action leads to neuronal cell death. The deposits, also called amyloid plaques, are formed around the neuropil. The exact molecular mechanism is still unclear. Abeta is a proteolytic cleavage product, by APP-cleaving enzyme 1 (BACE1) and beta-secretase complex, of membrane bound amyloid precursor protein (APP). Amyloid is the term used for proteins that are misfolded into a cross beta-sheet structure. Abeta is produced by various cell types and is secreted in the interstitial fluid. There are multiple cleavage sites in Abeta domain leading to various fragments of 36-43aa in length. The main peptide in the CSF terminate at residue 40. CSF contains many Abeta isoforms, of which Abeta40 is ~10 times more abundant than Abeta42. Abeta40 levels are unchanged in AD, but there is a change in Ab40:Abeta42 ratio in AD. This ratio has been thought of diagnostic importance. Assessment of Abeta in brain tissue is essential for diagnosis and is crucial for investigating the molecular mechanisms underlying AD. The antibody detects all forms of Abeta deposits without the need for formic acid pre-treatment on paraffin coupes and can be applied in a double staining strategy, making it suitable for investigating co-localization. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: The antibody is very specific and sensitive for the last C-terminal amino acid of synthetic Abeta1-40. No cross-reactivity was seen with other Abeta forms. Immunohistochemistry (paraffin): Fixation is performed using 4% paraformaldehyde, can be used for double staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice