CNP (CNPase, 2’, 3’-Cyclic Nucleotide 3’-Phosophodiesterase)

Size

£576.00

SKU:: 352679
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full length recombinant protein corresponding to human CNP/CNPase, expressed in E. coli.
Clone:: 1H10
Format:: Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol.
Extra Details:: The 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNP), is an enzyme which catalyzes the hydrolysis of 2′, 3′-cyclic nucleotides to 2′-nucleotides, These cyclic nucleotides are structurally different from the better known and studied 3′-5′-cyclic nucleotides of which the best known example is cyclic AMP. CNP (2’, 3’-Cyclic Nucleotide 3’-Phosophodiesterase) has two isoforms, CNPase1 (46kD) and CNPase2 (48kD), which are encoded separately by different promoters of the same gene. CNP is found in high levels in the brain and peripheral nerve, almost exclusively in oligodendrocytes and Schwann cells. CNP is the third most abundant protein found in the central nervous system myelin. It is found early in oligodendrocyte development and continues to be found in adult animals. It may play a role in RNA metabolism in the myelinating cell. CNP antibody can be used as a marker for oligodendrocyte and Schwann cells. CNP levels have been found to greatly reduced in various diseases, neurological mutant and in experimental conditions in which myelin is reduced. Additionally, reduced CNP brain levels have been reported in Down syndrome and Alzheimer’s disease. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: (IC) 1:500 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice