TESPA1, NT (KIAA0748, HSPC257, Protein TESPA1, Thymocyte-expressed positive selection- associated protein 1)

Size

£655.00

SKU:: 352579
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to 18aa near the N-terminal of human TESPA1.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1). TESPA1 encodes a protein of 458aa and is highly conserved among vertebrate species (2). TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3). TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1,4). Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 20ug/mL. Western Blot: 1-2ug/ml Immunocytochemistry: 5ug/mL. Optimal dilutions to be determined by the researcher. Positive Control: A431 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.