352554
SREBF1 (SREBP1, SREBP-1c, BHLHD1, Sterol Regulatory Element-Binding Protein 1, Class D Basic Helix-Loop-Helix Protein 1, SREBP-1)
Size
£655.00
- SKU:
- 352554
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 0°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to 17aa from the center of human SREBF1, located within amino acids 450 - 500.
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- The sterol regulatory element binding transcription factor 1 (SREBF1) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1). The related protein SREBF2 also binds SRE1 and activates transcription in an additive fashion to SREBF1 (2). SREBF1 is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1 (3). The SREBF1 proteins are important in the regulation of genes involved in lipid metabolism, while SREBF2 has been more closely associated with cholesterol synthesis and accumulation (4). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 20ug/ml Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Daudi Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice