352434
Phosphothreonine (PT)
Size
£838.00
- SKU:
- 352434
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Phosphothreonine conjugated to KLH.
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- Protein phosphorylation is a complex biochemical process that ultimately influences the mechanism by which a cell responds to a particular stimulus. A class of enzymes referred to as protein kinases regulates this process; these enzymes catalyze the transfer of the gama-P of ATP to a recipient protein that acts as a substrate. This post-translational modification is primarily directed onto serine and threonine amino acid residues within a protein. It has been noted that changes in the phosphorylation status of these phosphoproteins can alter the biological function of a protein. Additionally, aberrations in signal transduction cascade have been linked to a number of diseases including cancer, diabetes, heart disease, inflammation and neurological disorders. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:500-1:1000 Western Blot: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Rat Liver Tissue Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice