Orexin B (OX, PPORX, PPOX, Orexin, Hypocretin, Hcrt, NRCLP1)

Size

£659.00

SKU:: 352416
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Chicken/Avian
Immunogen:: Peptide corresponding to aa1-14 from human Orexin B.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (1). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130aa long peptide with a putative 33aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (1). These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:5000 (detection), 1:200-1:1000 (coating) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.