IQSEC1, NT (IQ Motif and Sec7 Domain 1, ADP- Ribosylation Factors Guanine Nucleotide-Exchange Protein 100, ARFGEP100, BRAG2, Brefeldin-Resistant Arf-GEF 2 Protein)

Size

£655.00

SKU:: 352312
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to 19aa from human IQSEC1 at N-terminal.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain. As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumor metastasis. IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: A-20 Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.